A transcriptome atlas of rabbit revealed by PacBio single-molecule long-read sequencing

A transcriptome atlas of rabbit revealed by PacBio single-molecule long-read sequencing

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Abstract It is widely acknowledged that transcriptional diversity largely contributes to biological regulation in eukaryotes.Since the advent of second-generation sequencing technologies, a large number of RNA sequencing studies have considerably improved our understanding of transcriptome complexity.However, it still remains a huge challenge for obtaining full-length transcripts because of difficulties in the short read-based assembly.In the present study we employ PacBio single-molecule long-read sequencing technology for whole-transcriptome profiling in iphone 13 pro max price winnipeg rabbit (Oryctolagus cuniculus).We totally obtain 36,186 high-confidence transcripts from 14,474 genic loci, among which more than 23% of genic loci and 66% of isoforms have not been annotated yet within the current reference genome.

Furthermore, about 17% of transcripts iphone 13 atlanta are computationally revealed to be non-coding RNAs.Up to 24,797 alternative splicing (AS) and 11,184 alternative polyadenylation (APA) events are detected within this de novo constructed transcriptome, respectively.The results provide a comprehensive set of reference transcripts and hence contribute to the improved annotation of rabbit genome.